Postdoctoral research fellowship : Start date April 1st, 2019
  • Job
  • Team Charles / Blank
  • 12/12/2018
  • A pourvoir
Détails de l'offre

 Postdoctoral Research Fellowship in Renal Immunopathology

 Job description

 A 3-year postdoctoral position (1+2) to study lupus nephritis pathophysiology is available in the Basophil, Mast cell and Immunopathology (BMI) team of the Centre for Research on Inflammation (CRI, INSERM U1149 CNRS ERL8252 University of Paris, Laboratoire d’Excellence INFLAMEX) in close collaboration with the Glomerulonephritis and Immunoreceptors team (GLOMI) and the Nephrology department of the Bichat Hospital, University of Paris, in Paris, France. The expected starting date is April, 1st 2019.

 More about the position

The project will aim at defining molecular pathways activating immune cells during the pathogenesis of systemic lupus erythematosus and associated flares of the renal disease. The teams involved in this collaborative project have a long and productive history in deciphering various immune activation pathways in renal and non-renal autoimmune disorders (Charles et al., Immunity 2009, Nat Med 2010 and 2012; Dema et al., J Exp Med 2014; Ben Mkaddem et al., J Clin Invest 2014, Nat Commun 2017; Jourde-Chiche et al., Rheumatol 2017, Pellefigues et al., Nat Commun 2018). The successful candidate will work under the supervision of Dr Nicolas Charles (BMI, CRI), Dr Sanae Ben Mkaddem (GLOMI, CRI) and Pr Eric Daugas (BMI, CRI and Nephrology Dpt, Bichat Hospital, AP-HP). The project is based on a translational approach and will require the analysis of Human patient samples and the use of animal models. 

Qualification requirements and skills

 The candidate holds a PhD in immunology or related subjects, with a strong background in immunology and immune-related disorders.

Desired qualifications and skills:

  • Experience with flow cytometry and FACS data analysis
  • Experience with dissection and tissue processing in mouse models
  • Experience with cell sorting from biological samples
  • Experience with qPCR, Immunohistochemistry, Immunofluorescence on tissues
  • High level report writing and presentational skills

The application must include 

  • Cover letter (statement of motivation, summarizing scientific work and research interest)
  • CV (summarizing education, positions and other qualifying activity)
  • A complete list of publications with brief statements of candidate contributions
  • List of 2-3 references (name, relation to candidate, e-mail and phone number) 

Special emphasis will be placed on the documented qualifications, as well as the candidate’s motivation and personal suitability.

Descriptif d'emploi

Qualification requirements and skills

The candidate holds a PhD in immunology or related subjects, with a strong background in immunology and immune-related disorders.

Desired qualifications and skills:

  • Experience with flow cytometry and FACS data analysis
  • Experience with dissection and tissue processing in mouse models
  • Experience with cell sorting from biological samples
  • Experience with qPCR, Immunohistochemistry, Immunofluorescence on tissues
  • High level report writing and presentational skills

The application must include

  • Cover letter (statement of motivation, summarizing scientific work and research interest)
  • CV (summarizing education, positions and other qualifying activity)
  • A complete list of publications with brief statements of candidate contributions
  • List of 2-3 references (name, relation to candidate, e-mail and phone number)

Special emphasis will be placed on the documented qualifications, as well as the candidate’s motivation and personal suitability.

Structure affectation / contact

Nicolas CHARLES, MD, PhD,

UMR 1149 INSERM – University Paris Diderot

Bichat Medical School

16 rue Henri Huchard

F-75018 Paris

Contact :

Charles (nicolas.charles@inserm.fr), Dr Sanae BenMKaddem (sanae.benmkaddem@inserm.fr) and Pr Eric Daugas (eric.daugas@aphp.fr).

Présentation de l'équipe

Basophils, Mast cells and Immunopathology (BMI)
Nicolas Charles  & Ulrich Blank

Besides their well-known role in allergies, mast cells (MC) and basophils (Ba) are important orchestrators of the inflammatory process with possible beneficial and pathological consequences. The team’s project focuses on the role of mast cells and basophils in immunopathology mainly in a non-allergic context using both fundamental and clinical approaches with local clinical departments. The strategy is based on the identification of the receptors expressed on these cells, their signaling pathways and the mechanism controlling the subsequent release of inflammatory mediators and on the effect of these mediators on tissue function, particularly on kidney, in animal models and patient samples. Both approaches are tightly linked to the analysis of therapeutic/prognostic/diagnostic potential of each of these steps using in vitro and in vivo approaches. Our team is particularly focused on the study of the role of basophils in lupus nephritis and of mast cells in renal diseases and fibrosis development as well as in tissue transplantation. 

The major research axes developed in our team are the following:

(A) Study of the molecular mechanisms and effectors regulating inflammatory mediator secretion in mast cells and basophils. This includes the study of new receptors of basophil function as well as the study of early and late signaling events involved in degranulation and cytokine chemokine secretion (axes 1-3).

(B) Study of the role of mast cells and basophils in immunopathology. This includes the analysis of the role of mast cells and their interaction with other inflammatory cells in fibrosis development, acute kidney injury and transplantation. The crosstalk of basophils with other innate and adaptive immune cells in the development of autoimmune diseases (in particular systemic lupus erythematous) is studied. We also investigate the role of these two cell compartments in the amplification of lupus disease flares and severe cardiovascular outcomes in a translational approach (axes 4-6).