Presentation

Vessels in liver diseases

Team composition:

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“from left to right: Marion Tanguy, Johanne Poisson, Pierre-Emmanel Rautou, Tazime Issoufaly, Sara Thietart, Louise Biquard”

Team leader: Prof. Pierre-Emmanuel Rautou, MD, PhD pierre-emmanuel.rautou@inserm.fr

[lien vers le CV : http://cvscience.aviesan.fr/cv/906/pierre-emmanuel-rautou ]

Lab manager: Marion Tanguy marion.tanguy@inserm.fr

Post-doc : Dr Johanne Poisson, MD, PhD johanne.poisson@inserm.fr

PhD student: Louise Biquard louise.biquard@inserm.fr

Master students:

Tazime ISSOUFALY tazime.issoufaly@inserm.fr

Sara Thietart sara.thietart@inserm.fr

Role of vessels in liver diseases

Our team focuses on the role of vessels in liver diseases. We are interested in primary vascular liver diseases, which are rare (Budd-Chiari syndrome, extrahepatic portal vein thrombosis and porto-sinusoidal vascular disease), as well as in the role of vessels in frequent liver diseases, including steatohepatitis and cirrhosis.

Current directions:

-        Liver sinusoidal endothelial cell in liver diseases progression: Recent advances point to the gut and the visceral adipose tissue as key players in liver diseases, suggesting the release by these organs of mediators acting on the liver. Liver endothelial cells are among the first cells in contact with the blood derived from these organs. These cells form an active interface between the blood and other liver cells. The role of liver endothelial cells in liver diseases and their complications remains overlooked and is studied by our team.

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-        Extracellular vesicles in liver diseases: Extracellular vesicles are membrane vesicles released in the extracellular space by cells. They include apoptotic bodies, microvesicles, also known earlier as microparticles, and exosomes. Extracellular vesicles are able to convey information from one cell to another. We are currently determining the implication of extracellular vesicles in liver diseases progression and complications by analyzing their role in the communication between the liver and other organs and also within the liver between liver cells. Extracellular vesicles can also be used as biomarkers. We already demonstrated that hepatocyte microvesicles can predict outcome of patients with cirrhosis. We are now validating these findings in large cohorts of patients and are developing new strategies to detect extracellular vesicles of various origins to precisely reflect disease progression.

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-        Porto-sinusoidal vascular disease, previously called intrahepatic non cirrhotic portal hypertension or nodular regenerative hyperplasia, is a rare disease characterized by alterations involving the portal venules and/or the sinusoids leading in some patients to portal hypertension and its complications. We are investigating the pathophysiology of this disease as well as the outcome of patients with this disease.

List of the most important recent publications

  1. Andrade F, Shukla A, Bureau C, Senzolo M, D’Alteroche L, Heurgué A, Garcia-Pagan JC, Turon F, Oberti F, Tripathi D, Roux O, Ceccaldi PF, de Raucourt E, Payancé A, Valla D, Plessier A, Rautou PE; VALDIG investigators. Pregnancy in idiopathic non-cirrhotic portal hypertension: A multicentric study on maternal and fetal management and outcome. J Hepatol. 2018 Aug 21.
  2. Payancé A, Silva-Junior G, Bissonnette J, Tanguy M, Pasquet B, Levi C, Roux O, Nekachtali O, Baiges A, Hernández-Gea V, Laouénan C, Lebrec D, Albuquerque M, Paradis V, Moreau R, Valla D, Durand F, Boulanger CM, Garcia-Pagan JC, Rautou PE. Hepatocyte microvesicle levels improve prediction of mortality in patients with cirrhosis. Hepatology. 2018 Mar 30
  3. Vion AC*, Kheloufi M*, Hammoutene A, Poisson J, Lasselin J, Devue C, Pic I, Dupont N, Busse J, Starke K, Lafaurie-Janvore J, Barakatf AI, Loyer X, Souyri M, Viollet B, Julia P, Tedgui A, Codogno P, Boulanger CM*, Rautou PE*. Autophagy is required for endothelial cell alignment and atheroprotection under physiological blood flow. Proc Natl Acad Sci U S A. 2017 Oct 10;114(41):E8675-E8684
  4. Poisson J, Plessier A, Kiladjian JJ, Turon F, Cassinat B, Andreoli A, De Raucourt E, Goria O, Zekrini K, Bureau C, Lorre F, Cervantes F, Colomer D, Durand F, Garcia-Pagan JC, Casadevall N, Valla DC, Rautou PE*, Marzac C*; French national network for vascular liver diseases. Selective testing for calreticulin gene mutations in patients with splanchnic vein thrombosis: A prospective cohort study. J Hepatol. 2017 Sep;67(3):501-507
  5. Bissonnette J, Altamirano J, Devue C, Roux O, Payance A, Lebrec D, Bedossa P, Valla D, Durand F, Ait-Oufella H, Sancho-Bru P, Caballeria J, Boulanger CM, Bataller R, Rautou PE. Plasma cytokeratin-18 fragments are an accurate non-invasive tool for the diagnosis of severe alcoholic hepatitis. Hepatology. 2017 Aug;66(2):555-563
  6. Weiss E*, Rautou PE*, Fasseu M*, Giabicani M, de Chambrun M, Wan J, Minsart C, Gustot T, Couvineau A, Maiwall R, Hurtado-Nedelec M, Pilard N, Lebrec D, Valla D, Durand F, de la Grange P, Monteiro RC, Paugam-Burtz C, Lotersztajn S, Moreau R. Type I interferon signaling in systemic immune cells from patients with alcoholic cirrhosis and its association with outcome. J Hepatol. 2017 May;66(5):930-941 (
  7. Poisson J, Lemoinne S, Boulanger C, Durand F, Moreau R, Valla D, Rautou PE. Liver sinusoidal endothelial cells: physiology and role in liver diseases. J Hepatol. 2017 Jan;66(1):212-227.
  8. Rautou PE, Tatsumi K, Antoniak S, Owens AP 3rd, Sparkenbaugh E, Holle LA, Wolberg AS, Kopec AK, Pawlinski R, Luyendyk JP, Mackman N. Hepatocyte Tissue Factor Contributes to the Hypercoagulable State in a Mouse Model of Chronic Liver Injury. J Hepatol. 2016 Jan;64(1):53-9.
  9. Loyer X, Paradis V, Hénique C, Vion AC, Colnot N, Guerin CL, Devue C, On S, Scetbun J, Romain M, Paul JL, Rothenberg ME, Marcellin P, Durand F, Bedossa P, Prip-Buus C, Baugé E, Staels B, Boulanger CM, Tedgui A, Rautou PE. Liver microRNA-21 is overexpressed in non-alcoholic steatohepatitis and contributes to the disease in experimental models by inhibiting PPARα expression. Gut. 2016 Nov;65(11):1882-1894.
  10. Lemoinne S, Thabut D, Housset C, Moreau R, Valla D, Boulanger CM, Rautou PE. The emerging roles of microvesicles in liver diseases. Nat Rev Gastro Hepat. 2014 Jun;11(6):350-61
  11. Rautou PE, Bresson J, Sainte-Marie Y, Vion AC, Paradis V, Renard JM, Devue C, Heymes C, Letteron P, Elkrief L, Lebrec D, Valla D, Tedgui A, Moreau R, Boulanger CM. Abnormal Plasma Microparticles Impair Vasoconstrictor Responses in Patients with Cirrhosis. Gastroenterology. 2012 Jul;143(1):166-76.

You want to join our team as Post-doc, PhD or Master student, please contact us! [lien vers mail : pierre-emmanuel.rautou@inserm.fr]

http://cvscience.aviesan.fr/cv/906/pierre-emmanuel-rautou

 

 

Selected publications
  1. Andrade F, Shukla A, Bureau C, Senzolo M, D’Alteroche L, Heurgué A, Garcia-Pagan JC, Turon F, Oberti F, Tripathi D, Roux O, Ceccaldi PF, de Raucourt E, Payancé A, Valla D, Plessier A, Rautou PE; VALDIG investigators. Pregnancy in idiopathic non-cirrhotic portal hypertension: A multicentric study on maternal and fetal management and outcome. J Hepatol. 2018 Aug 21.
  2. Payancé A, Silva-Junior G, Bissonnette J, Tanguy M, Pasquet B, Levi C, Roux O, Nekachtali O, Baiges A, Hernández-Gea V, Laouénan C, Lebrec D, Albuquerque M, Paradis V, Moreau R, Valla D, Durand F, Boulanger CM, Garcia-Pagan JC, Rautou PE. Hepatocyte microvesicle levels improve prediction of mortality in patients with cirrhosis. Hepatology. 2018 Mar 30
  3. Vion AC*, Kheloufi M*, Hammoutene A, Poisson J, Lasselin J, Devue C, Pic I, Dupont N, Busse J, Starke K, Lafaurie-Janvore J, Barakatf AI, Loyer X, Souyri M, Viollet B, Julia P, Tedgui A, Codogno P, Boulanger CM*, Rautou PE*. Autophagy is required for endothelial cell alignment and atheroprotection under physiological blood flow. Proc Natl Acad Sci U S A. 2017 Oct 10;114(41):E8675-E8684
  4. Poisson J, Plessier A, Kiladjian JJ, Turon F, Cassinat B, Andreoli A, De Raucourt E, Goria O, Zekrini K, Bureau C, Lorre F, Cervantes F, Colomer D, Durand F, Garcia-Pagan JC, Casadevall N, Valla DC, Rautou PE*, Marzac C*; French national network for vascular liver diseases. Selective testing for calreticulin gene mutations in patients with splanchnic vein thrombosis: A prospective cohort study. J Hepatol. 2017 Sep;67(3):501-507
  5. Bissonnette J, Altamirano J, Devue C, Roux O, Payance A, Lebrec D, Bedossa P, Valla D, Durand F, Ait-Oufella H, Sancho-Bru P, Caballeria J, Boulanger CM, Bataller R, Rautou PE. Plasma cytokeratin-18 fragments are an accurate non-invasive tool for the diagnosis of severe alcoholic hepatitis. Hepatology. 2017 Aug;66(2):555-563
  6. Weiss E*, Rautou PE*, Fasseu M*, Giabicani M, de Chambrun M, Wan J, Minsart C, Gustot T, Couvineau A, Maiwall R, Hurtado-Nedelec M, Pilard N, Lebrec D, Valla D, Durand F, de la Grange P, Monteiro RC, Paugam-Burtz C, Lotersztajn S, Moreau R. Type I interferon signaling in systemic immune cells from patients with alcoholic cirrhosis and its association with outcome. J Hepatol. 2017 May;66(5):930-941 (
  7. Poisson J, Lemoinne S, Boulanger C, Durand F, Moreau R, Valla D, Rautou PE. Liver sinusoidal endothelial cells: physiology and role in liver diseases. J Hepatol. 2017 Jan;66(1):212-227. 
  8. Rautou PE, Tatsumi K, Antoniak S, Owens AP 3rd, Sparkenbaugh E, Holle LA, Wolberg AS, Kopec AK, Pawlinski R, Luyendyk JP, Mackman N. Hepatocyte Tissue Factor Contributes to the Hypercoagulable State in a Mouse Model of Chronic Liver Injury. J Hepatol. 2016 Jan;64(1):53-9. 
  9. Loyer X, Paradis V, Hénique C, Vion AC, Colnot N, Guerin CL, Devue C, On S, Scetbun J, Romain M, Paul JL, Rothenberg ME, Marcellin P, Durand F, Bedossa P, Prip-Buus C, Baugé E, Staels B, Boulanger CM, Tedgui A, Rautou PE. Liver microRNA-21 is overexpressed in non-alcoholic steatohepatitis and contributes to the disease in experimental models by inhibiting PPARα expression. Gut. 2016 Nov;65(11):1882-1894.
  10. Lemoinne S, Thabut D, Housset C, Moreau R, Valla D, Boulanger CM, Rautou PE. The emerging roles of microvesicles in liver diseases. Nat Rev Gastro Hepat. 2014 Jun;11(6):350-61
  11. Rautou PE, Bresson J, Sainte-Marie Y, Vion AC, Paradis V, Renard JM, Devue C, Heymes C, Letteron P, Elkrief L, Lebrec D, Valla D, Tedgui A, Moreau R, Boulanger CM. Abnormal Plasma Microparticles Impair Vasoconstrictor Responses in Patients with Cirrhosis. Gastroenterology. 2012 Jul;143(1):166-76.
Team members
Team leader Pierre-Emmanuel Rautou, MD, PhD
Researchers Pierre-Emmanuel Rautou, MD, PhD
 Postdoctoral fellows Dr Johanne Poisson, MD, PhD , Post doc
Doctoral students Louise Biquard
Engeneers & technicians Marion Tanguy, IE
 Theses students
Tazime Issoufaly
  Sara Thietart